Method and apparatus for eluting proteins in affinity membrane p

Details Method and apparatus for eluting proteins in affinity membrane p Method and apparatus for eluting proteins in affinity membrane p

1993-03-23

1994-05-10

Parr, Margaret

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is organic

436531, 436177, 436178, 21050021, 210634, 210639, 435180, 435182, G01N 33545, B01D 1508, B01D 6100

Patent

active

053106881

ABSTRACT:
This invention relates to methods and apparatus for carrying out affinity separations of proteins under conditions optimized for recovering biologically active protein. The method utilizes a membrane-bound ligand first to capture and separate a ligate from a fluid mixture and subsequently to release said ligate in purified form. The present methods enhance the yield of active ligate recovered in the process. The invention has particular relevance to the recovery and purification of proteins from mixtures.

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