Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1978-02-09
1979-11-27
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
422 52, 23927, C12K 104, C12K 110
Patent
active
041760077
ABSTRACT:
A method and apparatus for removing porphyrins from a fluid sample which are unrelated to the number of bacteria present in the sample and prior to combining the sample with luminol reagent to produce a light reaction. The method involves a pre-incubation of the sample with a dilute concentration of hydrogen peroxide which inactivates the interfering soluble porphyrins. Further, by delaying taking a light measurement for a predetermined time period after combining the hydrogen peroxide-treated water sample with a luminol reagent, the luminescence produced by the reaction of the luminol reagent with ions present in the solution, being short lived, will have died out so that only porphyrins within the bacteria which have been released by rupturing the cells with the sodium hydroxide in the luminol reagent, will be measured. The measurement thus obtained can then be related to the concentration of live and dead bacteria in the fluid sample.
REFERENCES:
patent: 3564588 (1971-02-01), Soli
patent: 3745090 (1973-07-01), Chappelle et al.
patent: 3756920 (1973-09-01), Kelbaugh et al.
patent: 3959081 (1976-05-01), Witz et al.
Frosch Robert A. Administrator of the National Aeronautics and Space
Jeffers Eldon L.
Thomas Richard R.
Manning John R.
Marnock Marvin J.
Matthews Marvin F.
Tanenholtz Alvin E.
Warden Robert J.
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