Classifying – separating – and assorting solids – Precedent preparation of items or materials to facilitate... – Sorting special items or sorting by methods and apparatus...
Patent
1979-05-16
1982-09-07
Rolla, Joseph J.
Classifying, separating, and assorting solids
Precedent preparation of items or materials to facilitate...
Sorting special items or sorting by methods and apparatus...
209579, 209588, 209932, 250222PC, 324 71CP, 356 72, B07C 5342
Patent
active
043479359
ABSTRACT:
An improved method of sorting biological cells in a conventional cell sorter apparatus includes generating a fluid jet containing cells to be sorted, measuring the distance between the centers of adjacent droplets in a zone thereof defined at the point where the fluid jet separates into descrete droplets, setting the distance between the center of a droplet in said separation zone and the position along said fluid jet at which the cell is optically sensed for specific characteristics to be an integral multiple of said center-to-center distance, and disabling a charger from electrically charging a specific droplet if a cell is detected by the optical sensor in a position wherein it will be in the neck area between droplets during droplet formation rather than within a predetermined distance from the droplet center.
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Merrill et al.; "Investigations in High-Precision Sorting"; 9-16-78; Revision 1.
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Herzenberg et al.; "Fluorescence-activated Cell Sorting"; Scientific American, Mar. 1976, pp. 108-117.
Clouse, Jr. Clifton E.
Denny James E.
Gaither Roger S.
Rolla Joseph J.
The United States of America as represented by the United States
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