Method and apparatus for electrophoretic DNA band isolation

Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering

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2041828, 2041801, C25B 900

Patent

active

053840224

ABSTRACT:
An electrophoresis system is used to segregate a molecular specimen, the apparatus comprising a container, a gel layer and a buffer solution resident in the container, the gel layer having a well for receiving the molecular specimen. Electrical contacts are resident in the container and apply a potential across the gel layer to enable migration of the molecular specimen along a migration path in the gel layer. A second well is positioned in the gel layer and downstream in the migration path. A cup is inserted into the second well, the cup having a rim which, when the cup is resident in the second well, is positioned below the migration path of the molecular specimen within the gel layer. A membrane is also placeable in the second well to prevent migration of the molecular specimen past the cup. The molecular specimen thereby migrates into the cup and is prevented by the membrane from further migration along the migration path. Aspiration of the contents of the cup enables recovery of the molecular specimen.

REFERENCES:
patent: 4964961 (1990-10-01), Brautigam et al.
patent: 5217591 (1993-06-01), Gombocz et al.
patent: 5217593 (1993-06-01), MacConnell
GeneCAPSULE.TM., Copyright 1994, Geno Technology, Inc.

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