Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1984-10-15
1988-01-05
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 34, 435 39, 436519, 436534, 436547, 436800, G01N 3353, G01N 33554, C12Q 104, C12Q 106
Patent
active
047176555
ABSTRACT:
A method of distinguishing multiple subpopulations of cells from a single sample of cells of a variety of types comprises labeling particles with two or more marking agents. These particles are marked in a plurality of different pre-selected ratios of the agents ranging between zero percent and one hundred percent of each agent. Each such agent has distinguishing, quantifiable marking characteristics. The differently labeled particles are mixed with cells suspected of having specific receptors for the differently labeled particles. Each cell is analyzed to determine the ratio of any two identifiable marking characteristics associated with each cell so that is can be classified in a subpopulation category if its ratio of marking characteristics is related to one of the pre-selected ratios of marking agents.
An apparatus for carrying out the above-described method is also within the purview of the present invention.
REFERENCES:
patent: 3916197 (1975-10-01), Fulwyler
patent: 3916205 (1975-10-01), Kleinerman
patent: 4169137 (1979-09-01), Hirschfeld
patent: 4243318 (1981-01-01), Stohr
patent: 4284412 (1981-08-01), Hansen et al.
patent: 4510244 (1985-04-01), Parks et al.
patent: 4599307 (1986-07-01), Saunders et al.
Becton Dickinson and Company
Deck Randall E.
Marantz Sidney
Rodrick Richard J.
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