Method and apparatus for discrimination between scattered excita

Details Method and apparatus for discrimination between scattered excita Method and apparatus for discrimination between scattered excita

1977-10-25

1980-04-15

Willis, Davis L.

Radiant energy

Luminophor irradiation

Methods

250461R, G01T 110, G01N 2138

Patent

active

041985672

ABSTRACT:
A gated, pulsed laser spectrofluorometer is provided analyzing subpicogram amounts of fluorescent substance in sample volumes of less than 10 .mu.l. This is accomplished by repetitively exciting the fluorescent sample with a subnanosecond, intensive excitation radiation pulse and by gating a fluorescence radiation detector output signal so that detection starts after the excitation radiation pulse has decayed to a negligible intensity and ends when the fluorescence radiation detector output signal caused by the fluorescence emission from the sample has decayed to a level comparable to the electrical noise level of the electronic measuring apparatus connected to said fluorescence radiation detector.

REFERENCES:
patent: 4006360 (1977-02-01), Mueller
patent: 4037961 (1977-07-01), Macemon
patent: 4055768 (1977-10-01), Bromberg
patent: 4058732 (1977-11-01), Wieder

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