Method and apparatus for diagnosing capillary leak

Surgery – Truss – Pad

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128659, 128630, A61B 600

Patent

active

057043582

ABSTRACT:
This invention provides a method for determining the presence of capillary leak in a subject which comprises (a) introducing into the subject's circulation a pharmaceutically-acceptable solution comprising biodegradable, nontoxic, macromolecules, which macromolecules are smaller than endothelial gaps in capillaries of a subject having capillary leak, and each of which macromolecules is labeled with a detectable marker; (b) obtaining a sample of blood from the subject; (c) quantitatively measuring the detectable marker in the sample; (d) repeating steps (b) and (c) at a plurality of suitably spaced points in time; and (e) comparing the quantities of detectable marker so measured so as to ascertain a change in the quantity of detectable marker in the subject's circulation over time, the change being indicative of the presence of capillary leak, thereby determining the presence of capillary leak in the subject. The subject invention also provides an apparatus for displaying the quantity of detectable marker in a subject's circulation over time after introducing into the subject's circulation a pharmaceutically-acceptable solution comprising biodegradable, nontoxic macromolecules, which macromolecules are smaller than endothelial gaps of capillaries in a subject having capillary leak, and each of which macromolecules is labeled with a detectable marker, which apparatus comprises (a) means for obtaining samples of blood from the subject at a plurality of points in time; (b) means for quantitatively measuring the detectable marker in the sample obtained at each time point; and (c) means for displaying the quantities so measured. The aforementioned apparatus of the subject invention is useful for diagnosing capillary leak.

REFERENCES:
patent: 4994444 (1991-02-01), Zikria
patent: 5024231 (1991-06-01), Fledschuh et al.
Fleck, A., et al., Increased Vascular Permeability: A Major Cause of Hypoalbuminaemia In Disease and Injury. The Lance (1985) 1 (8432): 781-784 (Exhibit C).
Shippy, C. R., et al., Reliability of Clinical Monitoring to Assess Blood Volume in Critically Ill Patients. Critical Care Medicine (1984) 12: 107-112 (Exhibit E).
Gosling, P., et al. Generalized Vascular Permeability and Pulmonary Function in Patients Following Serious Trauma. The Journal of Trauma (1994) 36: 477-481 (Exhibit D).
Shoemaker, W.C., Pathophysiology and Fluid Management of Postoperative and Post-Traumatic ARDS. Assessment of Blood Volume Deficit. Shoemaker, W. C., et al. eds. Textbook of Critical Care. Philadelpphia: Saunders (1989), p. 626 (Exhibit F).
Shoemaker, W. C., et al. eds. Textbook of Critical Care. Philadelphia: Saunders (1989), p. 626 (Exhibit F).
Valeri, C.R., et al. Limitations of Measuring Blood Volume With Iodinated I 125 Serum Albumin. Archives of Internal Medicine (1973) 132: 534-538 (Exhibit G).
Volemetron. Pathol. Biol. (1967) 15 (9): 566-567 (Exhibit H).

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