Method and apparatus for determining the rate of enzyme reaction

Details Method and apparatus for determining the rate of enzyme reaction Method and apparatus for determining the rate of enzyme reaction

1978-04-26

1980-09-23

Warden, Robert J.

Chemistry: molecular biology and microbiology

Condition responsive control process

23230B, 356409, 356436, 364497, 364499, 364500, 422 68, 435 4, 435288, 435290, 435291, C12Q 300, C12M 140, C12M 138

Patent

active

042244056

ABSTRACT:
The rate of enzyme reaction is determined by determining of the quantity of enzyme, and deriving the rate of reaction per unit time of a reaction solution having an absorbance which is proportional to the time. A temperature correction coefficient which is used during the determination of the enzyme quantity is derived by temperature information which is supplied by a temperature sensor disposed within the reaction solution to be determined. An apparatus used for the determination of the rate of reaction comprises a means for containing the reaction solution, a temperature sensor directly disposed within the solution, a photoelectric transducer element responsive to light transmitting through the solution to determine the optical density of the solution, a logarithmic conversion circuit which converts the signal from the element into a corresponding absorbance signal, and a coefficient conversion circuit which is responsive to the combination of the absorbance signal and temperature information from the temperature sensor to correct a temperature coefficient.

REFERENCES:
patent: 3554654 (1971-01-01), Paatzsch et al.
patent: 3578404 (1971-05-01), Walles et al.
patent: 3706499 (1972-12-01), Rapoza et al.
patent: 3844661 (1974-10-01), Birkett et al.
patent: 3878049 (1975-04-01), Tannenbaum et al.
patent: 4012199 (1977-03-01), Luzer

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