Optics: measuring and testing – By particle light scattering – With photocell detection
Patent
1986-04-04
1991-02-05
Rosenberger, Richard A.
Optics: measuring and testing
By particle light scattering
With photocell detection
356 39, G01N 2153
Patent
active
049899783
ABSTRACT:
A method and apparatus for accurately determining the count per unit volume of particles, such as red blood cells, suspended in a fluid containing at least one undesirable interference particle, such as air bubbles. A predetermined volume of fluid containing the first and second particles is passed through the a flow cytometer which produces a forward light scattering pattern that is detected and measured. The volume and index refraction of each of the first and second particles is determined. The first and second particles are discriminated in accordance with their respective indices of refraction and the total count and volume of particles is corrected to provide the count per unit volume of red blood cells.
REFERENCES:
patent: 3549994 (1970-12-01), Rothermal et al.
patent: 3740143 (1973-06-01), Groner et al.
patent: 4429995 (1984-02-01), Goulas
patent: 4548500 (1985-10-01), Wyatt et al.
patent: 4577964 (1986-03-01), Hansen, Jr.
patent: 4735504 (1988-04-01), Tycko
Tycko et al., "Flow-Cytometric Light Scattering Measurement of Red Blood Cell Volume and Hemoglobin Concentration" Applied Optics., vol. 24, No. 9, May 1985, pp. 1355-1365.
Rosenberger Richard A.
Technicon Instruments Corporation
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