Method and apparatus for detection of unsuitable conditions for

Image analysis – Applications – Biomedical applications

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382133, 3644131, 364555, 435 72, 359397, G06K 900

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057153272

ABSTRACT:
A method and apparatus for determining whether a slide is suitable for processing. A suite of suitability tests are performed by an automated microscope system. The tests include magnification error flags, staining flags, main optical density flags, including detected intermediate cell nuclei, rings around detected intermediate cell nuclei, average texture measure of detected intermediate cell nuclei, average contrast to detected intermediate cell nuclei to cytoplasm, standard deviation of detected intermediate cell nuclei optical densities, detected intermediate cell ratios, average stripe area, measure of a saturated magnification of the image, measure of a grossly saturated magnification, and the percentage of images focused properly on a first try, including images never focused properly. The automated microscope quantifies the measurements in a reliable and repeatable way.

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