Method and apparatus for detection and quantitation of bacteria

Details Method and apparatus for detection and quantitation of bacteria Method and apparatus for detection and quantitation of bacteria

1989-10-25

1992-01-14

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 5, 435 29, 435 34, 435 39, 435173, 435287, 435311, 422101, 424 3, 424 71, 210474, 210477, 210455, 210505, C12Q 124, C12M 134

Patent

active

050810170

ABSTRACT:
A bacterial staining composition, process and apparatus for detection and quantitation of gram negative and gram positive bacteria in fluid samples are disclosed. The composition comprises a net negatively charged filter onto which bacteria are adsorbed and concentrated, an acid diluent which alters the electrostatic charge of the bacteria, thus enabling adsorption of the bacteria to the filter, a bacterial stain operative at about pH 8 to 12, and a washing reagent which enhances the diffusion of free dye away from the immobilized, stained bacterial. The color intensity at the center of the filter surface manifests the stained bacteria. It is compared to a standard color guide to determine the number of bacterial present in the fluid sample.

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