Method and apparatus for detecting molecules in solutions

Textiles: fiber preparation

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23230B, 23253R, 195127, 204195B, 204 1T, 424 12, G01N 2110, G01N 3316

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active

039752380

ABSTRACT:
A method and apparatus for optically detecting complexing of molecules in solution with surface-bound enzymes comprises slowly varying, in periodic fashion, the surface electrical potential so as to vary correspondingly the pH of the solution at the enzymatic sites through a range in which the Michaelis constant exhibits a relatively large change with pH variation. The potential is also oscillated over a smaller voltage range at a higher frequency. Phase sensitive detection of the output signal of an ellipsometer directed onto the surface provides a signal proportional to the concentration of complexing molecules.

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E. E. Uzgiris et al., Rev. Sci. Instrum., 45, 120 (1974).
Chemical Abstracts, 78: 39972d (1973).

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