Method and apparatus for detecting immunologic reactions by diff

Details Method and apparatus for detecting immunologic reactions by diff Method and apparatus for detecting immunologic reactions by diff

1974-04-01

1976-06-01

Wolk, Morris C.

Chemistry: physical processes

Physical processes

Crystallization

23253R, 23259, 23292, 1951035R, 424 12, G01N 3316, G01N 3102, C12K 100

Patent

active

039604900

ABSTRACT:
A thin layer of gel on a metallized solid surface has two or more wells formed through the gel which are subsequently filled with specimens of first and second solutions suspected of respectively containing first and second immunologically reactive biological particles specific to each other. The specmens are allowed to diffuse in the gel, and presence of the first and second biological particles in the solutions forms a complexed protein precipitate line on the metallized solid surface corresponding to the region of intersection of the two diffused specimens and which is visible with good contrast to the unaided eye without the need for staining the gel and provides a durable record of the immunological reaction which forms the precipitate.

REFERENCES:
patent: 3389966 (1968-06-01), Saravis
patent: 3390962 (1968-07-01), Goldsmith
patent: 3645687 (1972-02-01), Nerenberg
patent: 3674438 (1972-07-01), Shen
patent: 3692491 (1972-09-01), Trentelman
patent: 3709661 (1973-01-01), Hubscher
patent: 3718436 (1973-02-01), Ushakoff
patent: 3725004 (1973-04-01), Johnson
patent: 3791930 (1974-02-01), Saxholm
patent: 3843450 (1974-10-01), Saxholm
patent: 3853467 (1974-12-01), Giaever
patent: 3905767 (1975-09-01), Morris
A. Rothen et al., Helvetica Chimica Acta, 54, pp. 1208-1217, (1971).

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