Method and apparatus for detecting denaturation of nucleic acid

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing liquid or solid sample

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422 681, 422 50, 422 52, 422 55, 422 57, 422 8209, 435 6, 435 912, 4352872, 4352831, 4352881, 4352891, 4352901, 4352904, 356344, 73 6158, 204452, G01N 2164

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058611240

ABSTRACT:
An apparatus for detecting denaturation of a nucleic acid, including: denaturation condition controlling means for controlling condition of denaturation under which a double-stranded nucleic acid is separated into a first single-stranded nucleic acid and a second single-stranded nucleic acid; excitation light irradiation means for irradiating the double-stranded nucleic acid before denaturation, and the first single-stranded nucleic acid and second single-stranded nucleic acid after the denaturation; fluorescence detection means for detecting fluorescence emission based on the excitation light irradiation; and processing means for receiving, storing and processing a signal supplied from the fluorescence detection means.

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Fischer et al, "DNA Fragments Differing by Single Base-Pair Substitutions are Separated in Denaturing Gradient Gels: Correspondence With Melting Theory", Proc. Natl. Acad. Sci. USA, vol. 80, pp. 1579-1583, Mar. 1983.
Woolford et al, "Simplified Procedures for Detection of Amplified DNA Using Fluorescent Label Incorporation and Reverse Probing", FEMS Microbiology Letters 99 (1992) pp. 311-316.
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Analytical Biochemistry 221, 00-00 (1994) Assay of DNA Denaturation by Polymerase Chain Reaction-Driven Fluorescent Label Incorporation and Fluorescence Resonance Energy Transfer, Makiko Hiyoshi et al pp. 1-6.
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