Method and apparatus for detecting biological activity

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195 54, 195127, 195139, C12K 104

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039444716

ABSTRACT:
A sample of materials such as blood, urine, spinal fluid, or the like to be tested for the presence of biological activity is placed into a first sterile container with a suitable growth medium which includes a .sup.14 C carbon containing carbon source (such as .sup.14 C substituted glucose) which is fermentable to produce gaseous .sup.14 CO.sub.2. This first container is placed within a second container which also contains CO.sub.2 -absorbing material associated with a scintillation compound. The gaseous .sup.14 CO.sub.2 generated in the first container diffuses into the outer container where it is absorbed and held in contact with the scintillation compound. The decay of .sup.14 C will cause the scintillation compounds to emit light flashes which can be detected by a scintillation counter. Thus, the presence of .sup.14 CO.sub.2 is detected in the same container in which the reaction is taking place.

REFERENCES:
patent: 3676679 (1972-07-01), Waters

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