Coating processes – Plant member or animal specimen coating
Patent
1983-08-23
1985-04-09
Lusignan, Michael R.
Coating processes
Plant member or animal specimen coating
34 5, 62 62, 62100, 118 50, 378208, 424 3, 427 541, F26B 506, F26B 510, A01G 506, A01N 100
Patent
active
045101698
ABSTRACT:
This invention relates to a method for the cryopreparation of biological tissue samples for ultrastructural analysis. The process comprises vitrifying a biological tissue sample under severely rapid conditions and then depressurized. The depressurized, vitrified tissue sample is brought to equilibrium at a temperature of less than -140.degree. C. The tissue sample is then dehydrated while maintained in a state of thermal equilibrium. After reaching equilibrium the tissue sample is infiltrated with a degassed resin and followed by a polymerization of the resin to form an embedded tissue sample. This invention additionally relates to a sample holder apparatus which has unique use characteristics in the process of this invention.
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Lusignan Michael R.
The Board of Regents, The University of Texas
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