Method and apparatus for cryopreparing biological tissue

Drying and gas or vapor contact with solids – Apparatus – With means to treat gas or vapor

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34 92, 62268, 62100, F26B 506

Patent

active

051540070

ABSTRACT:
This invention relates to an apparatus and method for distillation drying of one or more biological samples. The drying apparatus includes a retaining assembly, a vacuum assembly, cooling means, monitoring means and control means for actively regulating the temperature and pressure conditions of biological tissue so that such tissue may be dried without substantial ultrastructural damage.

REFERENCES:
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patent: 4510169 (1985-04-01), Linner
patent: 4567847 (1986-02-01), Linner
patent: 4676070 (1987-06-01), Linner
patent: 4707998 (1987-11-01), Linner et al.
patent: 4745771 (1988-05-01), Linner et al.
patent: 4807442 (1989-02-01), Linner et al.
Louis Terracio and Karl G. Schwabe, Freezing and Drying of Biological Tissues for Electron Microscopy, Journal of Histochemistry and Cytochemistry vol. 29, No. 9, pp. 1021-1028 (1981).
U. B. Sleytr and A. W. Robards, Understanding the Artifac Problem in Freeze-Fracture Replication: A Review, The Royal Microscopical Society, pp. 103-123 (1982).
J. G. Linner, et al., A New Technique for Removal of Amorphous Phase Tissue Water Without Ice Crystal Damage: A Preparative Method for Ultrastructural Analysis and Immunoelectron Microscope, The Journal of Histochemistry and Cytochemistry, vol. 00, No. 0 (1986).

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