Method and apparatus for covalent immobilization of charge- conj

Details Method and apparatus for covalent immobilization of charge- conj Method and apparatus for covalent immobilization of charge- conj

1993-11-24

1996-08-06

Dawson, Robert A.

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

21050036, 21050037, 21050041, 2105021, 210506, 204462, 436 94, 436172, 436178, B01D 6138, C07H 106, G01N 2726

Patent

active

055430546

ABSTRACT:
A method for covalent immobilization of a carbohydrate molecule with an oppositely charged surface, comprising the steps of adsorbing the carbohydrate molecule to the oppositely charged surface in proximity to reactive moieties bound to the charged surface; and next activating the bound moiety sufficiently for covalent attachment to the adsorbed carbohydrate molecule. Also disclosed is a hydrophobic microporous polymer membrane coated with a cross-linked, cationic polymer having fixed charges thereon, the coating having enhanced epoxide content for covalent immobilization of the oppositely-charged carbohydrate molecule. A novel method for sequencing carbohydrates by covalent attachment of a carbohydrate conjugate to the membrane, and subsequent treatment with glycosidases, is also presented.

REFERENCES:
patent: 4512896 (1985-04-01), Gershoni
patent: 4617124 (1986-10-01), Pall et al.
patent: 4778601 (1988-10-01), Lopatin et al.
patent: 5004543 (1991-04-01), Pluskal et al.
patent: 5019231 (1991-05-01), Brandley et al.
patent: 5071909 (1991-12-01), Pappin et al.
patent: 5087337 (1992-02-01), Brandley et al.
patent: 5094731 (1992-03-01), Brandley et al.
Bjerrum, Ole J., et al, Electrophoresis 8:388-397 (1987).
Church, George M., et al., PNAS (USA) 81: 1991-1995 (1984).
Demeulemester, Claude, et al., Electrophoresis 8: 71-73 (1987).
Edge, Cristopher, et al., Nature 358: 693-694 (1992).
Lee, K. B., et al., Anal. Biochem. 205: 108-114 (1992).
Lee, Kyung-Bok, et al., Carbohydrate Res. 214: 155-168 (1991).
Merkle, R. K. et al., Methods in Enzynology 138: 232-250 (1987).
Osawa, T., et al., Ann. Rev. Biochem. 56: 21-42 (1987).
Van de Sluis, P. J. et al., J. Immun. Methods 104: 65-71 (1987).

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