Method and apparatus for concentrating and stabilizing...

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Cyclopentanohydrophenanthrene ring system doai

Reexamination Certificate

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Reexamination Certificate

active

07081451

ABSTRACT:
A method and apparatus for concentrating and stabilizing conjugated estrogens from pregnant mare urine on solid adsorbent supports to obtain a starting material for pharmaceuticals that contain a natural mixture of conjugated estrogens as active ingredient. The apparatus is suitable for decentralized concentration and stabilization of conjugated estrogen mixtures on cartridges in the vicinity of the horses. The adsorber cartridges can be loaded at the site of urine collection so it is unnecessary to transport large volumes of urine to a central processing point. The loading process can take continuously over a period of weeks until the column is saturated. The effluent urine remains at the site where facilities for its disposal are available. Only the loaded cartridge is transported, and transport can occur at longer intervals of up to several weeks. The stability of the conjugated estrogens on the adsorber assures there is no risk of decomposition.

REFERENCES:
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Heikkinen et al., “Reversed-Phase C18 cartridge for extraction of estrogens from urine and plasma.” Clin. Chem., vol. 27(7), pp. 1186-1189, 1981.
Shacketon et al., “Use of sep-pak cartridges for urinary steroid extraction: Evaluation of the method for use prior to gas chromatographic analysis.” Clinica Chimica Acta, vol. 107, pp. 231-243, 1980.
Risto Heikkinen, et al., “Reversed-Phase C18Cartridge for Extraction for Estrogens from Urine and Plasma” Clin. Chem., vol. 27, No. 7, pp. 118-1789, 1981.
C.H.L. Shacketon, et al., “Use of Sep-pak® Cartridges for Urinary Steroid Extraction: Evaluation of the Method for Use Prior to Gas Chromatographic Analysis” Clinica Chimica Acta, vol. 107, 1980, pp. 231-243.
H. Leon Bradlow, “Extraction of Steroid Conjugates with a Neutral Resin” Steroids, vol. 11, 1968, pp. 265-272.
Examination Report from Intellectual Property Office of New Zealand dated Jun. 10, 2003.

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