Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of co-culturing cells
Patent
1996-09-09
1998-06-09
Naff, David M.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of co-culturing cells
4352971, 4353041, 4353052, 4353053, C12N 500, C12M 304, C12M 306
Patent
active
057632758
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND OF THE INVENTION
The invention relates to a method of cell culturing in which a cell product released from a first cell culture is transported into a second cell culture and is integrated with the cells of the second cell culture.
This type of method may be used, for example, to transfer a cell product, such as a vector, from a first cell culture to a second cell culture, such as a lymphocyte culture. For this purpose, the first cell culture, for example a murine cell culture, which produces the vector of interest, will be cultured in a culture vessel. The cells will then be separated by centrifugation from the released vectors and the supernatant containing the vector will be added to the second cell culture, the lymnphocytes. In order to avoid thinning the lymphocyte culture too much, the addition of vectors is repeated several times. It is also necessary to keep in mind that the half-life of the vectors lasts only 10 minutes, which, on the one hand, requires working as quickly as possible, and on the other hand, excludes the addition of large quantities of the vector in a single operation. For this reason, the known methods are slow.
In addition, because of the large amount of equipment required and numerous steps involved in using the known methods, there is a constant risk of contamination to the cell culture.
Moreover, the invention relates to a culture vessel for cell cultures having a first cell culture chamber for the reception of a first cell culture, having a supply chamber for the reception of a culture medium, having a dialysis membrane between the cell culture chamber and the supply chamber by means of which the nutrients are transported into the cell culture chamber and the metabolic products of the cell culture are removed from the cell culture chamber, and having a gas-exchange membrane that forms at least part of the external portion of the culture vessel.
This type of culture vessel is known from DE-Al 42 29 325. It describes a culture vessel which has a supply chamber and at least one cell culture chamber within a substantially hollow, cylindrical plastic housing. The two chambers are separated from one another by a flat, semipermeable dialysis membrane. The culture medium is transported from the supply chamber into the cell culture chamber and the metabolic products removed from the cell culture chamber by means of the dialysis membrane. The end surface of the plastic tube facing the cell culture chamber is closed by a silicone foil approximately 0.5 mm in diameter that serves as a gas-exchange membrane. The silicone foil is permeable to oxygen and carbon dioxide, but impermeable to liquids and bacteria.
The known culture vessel is able to rotate on a roller-rotator device on its long axis, by which means mixing of the liquid culture medium and cell culture plaque takes place in the cell culture chamber Oxygen supply to the cell culture is effected through the direct passage of oxygen via the gas-exchange membrane to the cell culture plaque as well as indirectly via prior passage to the nutrient solution, from which it reaches the cell culture chamber via the dialysis membrane.
The known culture vessel may have several cell culture chambers. It is consequently capable of culturing one or more cell cultures. The previously described method, referred to as co-culturing, however, is not feasible by this means.
SUMMARY OF THE INVENTION
It is the object of this invention to offer a method for which the risk of contamination during transfer of a cell product to a second cell culture is reduced and which permits a high yield and rate of production. Furthermore, it is the object of this invention to provide a culture vessel for cell cultures, in which a co-culturing method may be implemented with a minimum of labor.
With regard to method, the object is solved in the context of the convention by the previously mentioned method, in that the first and second cell cultures are contiguous, and in that they are separated by a membrane which is impermeable to the cells of the cell cult
REFERENCES:
patent: 4661455 (1987-04-01), Hubbard
patent: 5173225 (1992-12-01), Range et al.
Falkenberg et al., A simple and inexpensive high density dialysis tubing cell culture system for the in vitro production of monoclonal antibodies in high concentration, J. Immunol. Methods, vol. 165, pp. 193-206, 1993.
Kopowski Eckart
Nagels Hans-Otto
Schroder Dieter
Heraeus Instruments GmbH
Kerr Janet M.
Naff David M.
LandOfFree
Method and apparatus for co-culturing cells does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method and apparatus for co-culturing cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and apparatus for co-culturing cells will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2198237