Method and apparatus for classifying B-lymphocytes

Details Method and apparatus for classifying B-lymphocytes Method and apparatus for classifying B-lymphocytes

2008-04-08

2008-04-08

Gabel, Gailene R. (Department: 1641)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S007250, C435S372200, C436S522000, C436S017000, C436S063000, C436S064000, C436S164000, C422S068100, C422S082050, C422S082090, C422S091000

Reexamination Certificate

active

07354727

ABSTRACT:
A B-lymphocyte classifying method includes the steps of (1) preparing a measurement sample by mixing a blood sample with a lysing reagent to lyse erythrocytes and to shrink the B-lymphocytes in the blood sample, (2) letting the measurement sample flow through a flow cell of a flow cytometer, (3) radiating a light beam onto a cell in the measurement sample that is flowing through the flow cell, (4) detecting at least two scattered light emitted from the irradiated cell, (5) specifying a region of a B-lymphocyte cluster in accordance with the detected scattered light, and (6) counting the number of B-lymphocytes in the specified region.

REFERENCES:
patent: 4284412 (1981-08-01), Hansen et al.
patent: 4987086 (1991-01-01), Brosnan et al.
patent: 5648225 (1997-07-01), Kim et al.

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