Chemistry: analytical and immunological testing – Automated chemical analysis – Utilizing a centrifuge or compartmented rotor
Reexamination Certificate
2007-11-13
2007-11-13
Soderquist, Arlen (Department: 1743)
Chemistry: analytical and immunological testing
Automated chemical analysis
Utilizing a centrifuge or compartmented rotor
C073S061650, C073S061660, C073S061690, C356S338000, C356S340000, C356S343000, C422S064000, C422S072000, C422S073000, C422S082050, C422S082090, C436S063000, C436S070000, C436S086000, C436S166000, C436S177000, C494S010000, C494S016000
Reexamination Certificate
active
11707835
ABSTRACT:
A method and apparatus is described by which means molecules in suspension may be characterized in terms of the size and mass distributions present. As a sample solution is separated by centrifugal means, it is illuminated at a particular radial distance from the axis of rotation by a fine, preferably monochromatic, light beam. Despite the high resolution of such devices, a key problem associated with most separators based upon use of centrifugal forces is the difficulty in deriving the absolute size and/or molar mass of the separating molecules. By integrating means to detect light scattered, over a range of scattering angles, from samples undergoing centrifugal separation, molecular sizes in the sub-micrometer range may be derived, even in the presence of diffusion. Adding a second light beam at a displaced rotational angle, preferably of an ultraviolet wavelength, that intersects the sample at the same radial region as the first beam permits determination of the molecular concentration at that region. Combining the light scattering data with the associated concentration permits the determination of the associated molar mass. In a preferred embodiment, the light beam and detectors may be controlled to scan synchronously the sample radially during separation.
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Soderquist Arlen
Wyatt Philip J.
Wyatt Technology Corporation
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