Method and apparatus for characterizing biologically active agen

Details Method and apparatus for characterizing biologically active agen Method and apparatus for characterizing biologically active agen

1975-05-27

1976-12-14

Monacell, A. Louis

Textiles: fiber preparation

195127, 195139, C12K 104

Patent

active

039974049

ABSTRACT:
A method for characterization of biological activity comprising providing a plurality of gas-tight cells each having a light-permeable wall section, each cell containing a different .sup.14 C-labeled fermentable substrate, a carbon dioxide absorber and a scintillation compound in intimate proximity to each other remote from said substrate, and an axenic sample of a biologically active material; subjecting said cells to conditions conducive to biological activity and thereafter measuring the light emitted by the scintillation compound as a result of the decay of .sup.14 CO.sub.2 generated by the metabolic action of the biologically active agent in said sample on said substrate and captured by the carbon dioxide absorber. Apparatus is provided comprising a plurality of sterile chambers containing different .sup.14 C-labeled fermentable substrates, means to facilitate introduction of an axenic sample of a biologically active agent into each chamber, means for sealing each chamber to form a gas-tight cell having a light-permeable wall section, and a carbon dioxide absorber and a scintillation compound in intimate proximity to each other in each cell adjacent the light-permeable wall section.

REFERENCES:
patent: 2914447 (1959-11-01), Levin
patent: 3506402 (1970-04-01), Simon
patent: 3676679 (1972-07-01), Waters
patent: 3819489 (1974-06-01), Kronick et al.
patent: 3844894 (1974-10-01), Kronick et al.

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