Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1989-06-22
1992-01-07
Naff, David M.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435174, 435176, 43524024, 435284, 435285, 435286, 435288, 435819, C12N 500, C12N 1114, C12N 300, C12N 1100
Patent
active
050791613
ABSTRACT:
Cell cultivation is carried out by proliferating cells in a small preliminary culture tank containing immobilizing carriers, stripping proliferated cells from the carriers, forming a cell suspension of the stripped cells in culture medium in an intermediate reservoir, intermittently transferring the suspension into a main culture tank containing ceramic immobilizing carriers and further culturing the cells. The main culture tank has an inversed funnel-shaped inlet above the carriers with a perforated plate attached to the bottom of the inlet for uniform distribution of cell suspension from above the carriers, and a plate in the form of a net under the carriers on a perforated plate for uniform distribution of cell suspension from beneath the carriers. The main tank additionally has a lid detachably carrying the inversed funnel-shaped inlet an intermediate drum-shaped portion from which the lid is detachable and a lower portion detachably carrying the plate in the form of a net and being detachable from the drum-shaped portion. The lower portion is held on or separated from the drum-shaped portion by means of a link mechanism and a cylinder capable of lowering the lower portion to separate it from the drum-shaped portion.
REFERENCES:
Pharmacia Fine Chemicals, Microcarrier Cell Culture Principles and Methods, 1985, pp. 13-16, 24,25,34,35,44,45,49,50,55-6174 and 75.
Kobayashi Naoki
Kumazawa Eitaro
Matsuda Yoshiaki
Mitsuda Shinjiro
Sakuma Hideo
Naff David M.
Snow Brand Milk Products Co. Ltd.
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