Chemistry: molecular biology and microbiology – Apparatus – Differentiated tissue perfusion or preservation apparatus
Patent
1979-03-27
1980-12-23
Penland, R. B.
Chemistry: molecular biology and microbiology
Apparatus
Differentiated tissue perfusion or preservation apparatus
435283, 435285, 2103212, 2103213, C12M 300
Patent
active
042411874
ABSTRACT:
A method and apparatus for culturing biological cells and tissues in high density are disclosed, employing perfused blood as the nutrient source. The cells or tissues are separated from the perfused blood by means of a microporous membrane which is maintained in close contact with the cells or tissues, and which is permeable to plasma solutes and to cell products and impermeable to blood cellular components, whereby continuous diffusion of plasma solutes and cell products takes place between the perfused blood and the cells or tissues through the microporous membrane while maintaining the cells or tissues separated from the cellular components of the blood. The blood flow passageway across the surface of the microporous membrane is designed to enable blood perfusion at a sufficiently high linear velocity so as to avoid thrombosis. Continuous blood perfusion through the blood flow passageway is provided by connecting the blood flow passageway into an arterio-venous shunt constructed in a living donor animal.
REFERENCES:
patent: 2996429 (1961-08-01), Toulmin
patent: 3734851 (1973-05-01), Matsumura
patent: 3827565 (1974-08-01), Matsumura
patent: 3883393 (1975-05-01), Knazek et al.
patent: 3941662 (1976-03-01), Munder et al.
patent: 3993560 (1976-11-01), Halpern
Penland R. B.
United States of America
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