Chemistry: analytical and immunological testing – Heterocyclic carbon compound – Hetero-o
Patent
1993-08-13
1995-05-02
Redding, David A.
Chemistry: analytical and immunological testing
Heterocyclic carbon compound
Hetero-o
436161, 536 185, 536 221, 536124, 5361231, G01N 3366
Patent
active
054118921
ABSTRACT:
A method for analyzing carbohydrates involves solvolyzing the carbohydrate in anhydrous hydrogen fluoride. Solvolysis is terminated by the introduction of a carboxylic acid anhydride to the solvolyzed carbohydrate and hydrogen fluoride reaction mixture, resulting in the formation of acetic acid and acetic acid acyl fluoride. The acetic acid acyl fluoride reacts with hydroxyl groups on the solvolyzed carbohydrate to form peracylated carbohydrates. The resulting peracylated carbohydrates are dried to remove the solvent by-products, and can then be separated by gas chromatography. The gas chromatography eluents are then identified using mass spectrometer. An apparatus for automatically carrying out the solvolysis and carboxylic acid anhydride termination and acetylization steps is also provided.
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Mietchen et al., "From Isopropylidene Sugars to Acylated Pyranosyl or Furanosyl Fluorides in One Step", Synthesis (10), (1991) pp. 885-888.
Redding David A.
University of Washington
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