Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...
Patent
1997-12-10
1999-12-21
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Maintaining blood or sperm in a physiologically active state...
435 721, 435 723, 435 724, 435 725, 435372, 4353721, 4353722, 4353723, 210515, 436523, A01N 102, G01N 33567
Patent
active
060047437
ABSTRACT:
A cell population or subpopulation enrichment procedure for separating undesired populations or subpopulations from a biological sample utilizing relatively heavy, dense particles and gravity sedimentation. The particles have one or more reactants bound thereto which are specific to and will bind with the selected population or subpopulation to be eliminated from the sample. The particles preferably are mixed with the sample by repeatedly causing the particles to settle through a substantial portion of the sample to bind to the selected population. The particles with the bound selected population to be eliminated then are allowed to preferentially settle in the sample and the supernatant including the enriched population or subpopulation is separated from the particles with the population to be eliminated bound thereto. The enriched population supernatant can then be analyzed, utilized as is, have other populations or subpopulations removed or have additional amounts of remaining cells of the previously eliminated populations removed in additional steps.
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Kenyon Norma S.
Ricordi Camillo
Russell Thomas R.
Zwerner Robert K.
Lankford , Jr. Leon B.
Tate Christopher R.
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