Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1989-08-24
1994-06-28
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 3, 435289, 435968, 436518, 436 8, 436 50, 436172, 436800, 436805, 422 8208, 2504591, G01N 33535
Patent
active
053246357
ABSTRACT:
An analyzer has a reaction disk for holding a plurality of reaction containers and a fluorophotometer for measuring fluorescence stemming from solutions in the containers. Most of the containers contain solid phases attached with antibodies but at least one container does not contain any solid phase. In normal operation of the analyzer, a test sample containing antigens and a latently fluorescent reagent such as an antibody labeled by an enzyme are added to a container containing a solid phase. In this container, a fluorescent substance is created through an enzyme reaction. Light is irradiated on the container and the fluorescence emitted from the fluorescent substance is measured. While measuring test samples, fluorescence stemming from a reference sample, such as quinine sulfate, is measured to produce values for the reference sample by which measured values for the test samples are corrected. Prior to measuring general test samples by the analyzer, fluorescence stemming from a reference sample in a container removed of any solid phase is measured and compared with a setting value. If the results of the comparison indicate that the normal operation will be difficult to continue, then the sampling operation by the pipetter is discontinued.
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Hashimoto Hiroshi
Imai Kyoko
Kawase Masumi
Hitcahi, Ltd.
Kepplinger Esther L.
Wolski Susan
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