Method and apparatus for automated processing and aliquoting of

Chemistry: analytical and immunological testing – Biological cellular material tested

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436 45, 436177, 422 72, 2105121, 210787, 494 16, 494 17, B04B 502, B04B 1100, B01D 2126

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047404728

ABSTRACT:
A rotor and disc assembly for use in a centrifugal fast analyzer. The assembly is designed to process multiple samples of whole blood followed by aliquoting of the resultant serum into precisely measured samples for subsequent chemical analysis. The assembly requires minimal operator involvement with no mechanical pipetting. The system comprises (1) a whole blood sample disc, (2) a serum sample disc, (3) a sample preparation rotor, and (4) an analytical rotor. The blood sample disc and serum sample disc are designed with a plurality of precision bore capillary tubes arranged in a spoked array. Samples of blood are loaded into the blood sample disc in capillary tubes filled by capillary action and centrifugally discharged into cavities of the sample preparation rotor where separation of serum and solids is accomplished. The serum is loaded into the capillaries of the serum sample disc by capillary action and subsequently centrifugally expelled into cuvettes of the analytical rotor for analysis by conventional methods.

REFERENCES:
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patent: 3798459 (1974-03-01), Anderson et al.
patent: 3854508 (1974-12-01), Burtis et al.
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patent: 3890101 (1975-06-01), Tiffany et al.
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patent: 3899296 (1975-08-01), Mailen et al.
patent: 4052164 (1977-10-01), Konig
patent: 4169060 (1979-09-01), Columbus
Burtis et al., "The Development of Rotors Having Separate Sample and Reagent Transfer Channels for Use with Centrifugal Fast Analyzers", Analytical Letters, 7(8&9), 1974.
J. C. Mailen et al., "Techniques for Fabrication and Assembly of Rotors for Use in a Miniature Fast Analyzer", Analytical Letters, 6(3), 1973.
C. A. Burtis et al., "Optimization and Analytical Applications of the Technique of Dynamic Introduction of Liquids into Centrifugal Analyzers", Advanced Concepts, Clin. Chem., 20/8, 1974.

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