Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1983-11-15
1986-12-09
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
2504612, 435 29, 435287, 436519, 436800, 436805, 436807, 436808, 436809, 436821, G01N 3353, G01N 33554
Patent
active
046280260
ABSTRACT:
An automated system for rapid sequential photometric analysis of a collection of double fluorochrome stained lymphocyte specimens, useful for antibody screening or lymphocytotoxicity analysis. The specimens are sequentially alternately irradiated with light of two distinguishable wavelengths, producing fluorescence at two distinguishable wavelengths. The fluorescent emission light intensity for each irradiation of each specimen is measured using a photometer and computer. The computer controls the synchronization of the irradiation through alternately selected condenser sets with the sequential movement of specimens into the optical path of the irradiating and detected light, and calculates the quotient of the light intensities emitted from each specimen at the two selected fluorescent light wavelengths. These quotients are compared against a control ratio (for lymphocytotoxicity analysis) to classify the specimen. Also described is a method of preparing specimens for such analysis, which requires that a complement be added to the first staining solution after the latter is applied to the specimens, then this combination agitated, and then the second staining solution added and the specimen incubated.
REFERENCES:
patent: 4063816 (1977-12-01), Itoi
patent: 4100416 (1978-07-01), Hirshfeld
patent: 4265855 (1981-05-01), Mandle
patent: 4299796 (1981-11-01), Esch
patent: 4314026 (1982-02-01), Descamps-Latscha
patent: 4336029 (1982-06-01), Natale
patent: 4354114 (1982-10-01), Karnaukhov
patent: 4471056 (1984-09-01), Grumet
patent: 4499052 (1985-02-01), Fulwyler
patent: 4532203 (1985-07-01), Ullman
patent: 4554839 (1985-11-01), Hewett
patent: 4559309 (1985-12-01), Evenson
patent: 4581334 (1986-04-01), Kirchanski
Chemical Abstracts, 101:86887h (1984).
Abstract by Pablo Rubinstein, M.D. et al., entitled "Automated Reading of Cytotoxicity with the Contrast Fluorescence Test (CFT)", American Association for Clinical Histocompatibility Testing, Mar. 1981.
Rotman and Papermaster, (1966) Proc. N.A.S., pp. 134-141.
Edidin and Church, Transplantation, vol. 6, No. 9, pp. 1010-1014 (1968).
LePecq and Paoletti, J. Mol. Biol. (1967) 27, 87-106.
White, in Standardization in Immunofluorescence, Holborow Ed (1970).
Fong and Kissmeyer-Nielsen, Tissue Antigens (1972) 2.57-63.
Elves, Journal of Immunological Methods 2 (1972) 129-136.
Krisshan, The Journal of Cell Biology-vol. 66, 1975, pp. 188-193.
Goding, Journal of Immunological Methods, 13 (1976) 215-226.
Bruning, Kardol and Arentzen, Journal of Immunological Methods, 33 (1980) 33-44.
Edidin, The Journal of Immunology, vol. 104, No. 5, May 1970.
Tagasugi, Transplantation, vol. 12, No. 2, (1971).
Martel, Jaramillo, Allen and Rubinstein, Vox Sang., 27: 13-20 (1974).
van Rood, van Leeuwen and Ploem, Nature, vol. 262, pp. 795-797 (1976).
Bruning, Claas, Kardol, Lansbergen, Naipal and Tanke, Human Immunology, 5, 225-231 (1982).
Johnson, Goddard and Holborow, Journal of Immunological Methods, 50 (1982) 277-280.
van Lambalgen, Bruning and Bradley, Transplantation Proceedings, vol. XV, No. 1, pp. 69-71 (1983).
Zeiss brochure, "IM 35 ICM 405 Photo-Invertoscopes".
Leitz brochure, "MPV Compact MT".
Gardell Dietlind
Rock Gail
LandOfFree
Method and apparatus for automated double fluorochromization ana does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method and apparatus for automated double fluorochromization ana, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and apparatus for automated double fluorochromization ana will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1385335