Planting – Drilling and broadcasting – Main and auxiliary frame machines
Patent
1977-04-01
1978-10-24
Bagwill, Robert E.
Planting
Drilling and broadcasting
Main and auxiliary frame machines
195120, 195126, 215248, 215261, 47 11, 47 58, 47 59, A01G 100
Patent
active
041215257
ABSTRACT:
A method for aseptically sowing small seeds or spores of green plants includes preparation of a sterile closed flask (one-half pint jar) containing a growth medium and having both a contamination-resistant vent and a puncturable, resealing membrane in its closure. The seeds or spores are placed in a hypodermic syringe and a sterilizing solution is drawn into the syringe and held in contact with the seeds long enough to sterilize their surfaces. If desired, the sterilizing solution is ejected from the syringe through a filter or otherwise so as to retain the seeds or spores, and replaced by a wash liquid. The syringe is fitted with a sterile hypodermic needle of sufficient bore size to pass the seeds or spores. The needle is inserted through the membrane, after sterilizing the outside surface of the membrane, and the sterilized seeds or spores are discharged directly from the syringe onto the growth medium in the flask.
REFERENCES:
patent: 1072674 (1913-09-01), Tint
patent: 2153981 (1939-04-01), Heineman
patent: 2186908 (1940-01-01), Page et al.
patent: 2706702 (1955-04-01), Carski
patent: 2851821 (1958-09-01), Guiochon
A simple method . . ., Harrison, Amer. Orchid Soc. Bull., Aug. 1970, pp. 715-716 relied on.
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