Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose
Patent
1991-04-10
1993-09-28
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving glucose or galactose
435 22, 435288, 210632, C12Q 154, C12Q 140, C12M 140
Patent
active
052485978
ABSTRACT:
A method and apparatus for the determination of the average molecular weight, average chain length or dextrose equivalent of starch and related carbohydrates containing glucose units. The starch is analyzed by determining the free glucose amount (Gf) contained in starch or related carbohydrate sample, determining a gross glucose amount (Gt) rendered by hydrolysis of starch or related carbohydrates, and further determining a glucose amount (Gr) rendered by hydrolysis of a reduced product which is obtained by reduction of reducing terminals contained in starch or related carbohydrate.
The average molecular weight, average chain length or dextrose equivalent of the starch is determined using the formulas stated below from the measured amounts of the free glucose amount (Gf), and the glucose amount (Gr). The apparatus preferably utilized immobilized enzymes to conduct the hydrolysis of the starch sample, contains a device for recognizing and storing the measured values and computed data, and contains further a device for computing average molecular weight (Ma), average chain length (Dp) or dextrose equivalent value (DE) according to the following expressions: Dp=2.multidot.Gt/(Gt-Gr+Gf), Ma=18(19.multidot.Gt+Gf-Gr)/(Gt-Gr+Gf), DE=1000(Gt-Gr+Gf)/(19.multidot.Gt-Gr+Gf), or DE=1000/(9.multidot.Dp+1).
REFERENCES:
patent: 4102745 (1978-07-01), Thompson
Marro-Vega, G.A., Determination of Mono and Oligosacchar . . . Anal Chem 1989 61 831-838.
Marro-Vega G.A., Post-Column Derivaziation . . . Anal Chem Acta 234 (1990) 13-29.
Emneus J., Flow System for Starch Determination . . . Anal Chem 1990 62, 263-268.
Analytica Chimica Acta vol. 230, No. 1, Mar. 1, 1990, Amsterdam NL, pp. 75-82, T. Ideda et al., "Amperometric response to reducing carbohydrates of an enzyme electrode based on oligosaccharide dehydrogenase, Detection of lactose and alpha-amylase".
Hasizume Yoshio
Hayashi Ryuzo
Kariyone Akio
Gitomer Ralph
Kanzaki Paper Mfg. Co. Ltd.
Wityshyn Michael G.
LandOfFree
Method and apparatus for analyzing starch and related carbohydra does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method and apparatus for analyzing starch and related carbohydra, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and apparatus for analyzing starch and related carbohydra will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2190363