Method and apparatus for analyzing compounds with amino group

Chemistry: analytical and immunological testing – Peptide – protein or amino acid – Amino acid or sequencing procedure

Reexamination Certificate

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C436S096000, C436S173000, C250S281000, C422S070000

Reexamination Certificate

active

07494815

ABSTRACT:
The present invention provides a method and apparatus for analyzing compounds with amino group(s) contained in biological organisms. In this method, a compound with amino group(s) is derivatized and then the derivative of the compound with amino group(s) is eluted by liquid chromatography using a stepwise elution means on a concentration gradient. Subsequently, the derivative of the compound with amino group(s) eluted from the liquid chromatography is detected by mass spectrometry.

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