Method and apparatus for amplification of nucleic acid...

Details Method and apparatus for amplification of nucleic acid... Method and apparatus for amplification of nucleic acid...

2004-04-29

2009-02-10

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Apparatus

C435S091200, C435S091100

Reexamination Certificate

active

07488595

ABSTRACT:
The present invention generally relates to methods and apparatuses for amplifying nucleic acid sequences using immobilized DNA polymerase. More particularly, it relates to methods and apparatuses useful for amplifying target nucleic acid sequences by forming a plurality of reaction regions in which polymerase chain reaction (PCR) can occur, positioning immobilized DNA polymerase in a specific reaction region, and circulating DNA through the reaction regions. The present invention provides those methods and apparatuses that allow simple separation and recovery of the DNA polymerase after the amplification, that can be operated not only with thermostable DNA polymerases but also with non-thermostable DNA polymerases, and that are simpler in their designs and processes so that they can be readily integrated into complex devices such as Lab-on-a-chip.

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Kopp et al. “Chemical Amplification: Continuous-Flow PCR on a Chip” Science. May 15, 1998. vol. 280, pp. 1046-1048.

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