Method and an apparatus for differentiating a sample of biologic

Image analysis – Histogram processing – For setting a threshold

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382 18, 36441313, G06K 900, G06K 946, G06K 966, G06F 1500

Patent

active

051230550

ABSTRACT:
A and an apparatus method for quickly and easily identifying the five basic components of leukocyte cells: basophils, eosinophils, neutrophils, monocytes and lymphocytes is disclosed. The method measures the size of each of the cells. Computation of the quantile feature of the cells and their color is then made. BA and EO cells are differentiated from LY and MO and Ne cells using the present method. BA and EO cells can also be differentiated from LY, MO, and Ne cells using methods of the prior art. Once BA and EO cells have been differentiated from the five leukocyte cells, LY cells can be differentiated from MO and NE cells based upon the size of the LY cells. The average color intensity of the LY cells determined is then used as the threshold to differentiate MO cells from NE cells.

REFERENCES:
patent: 3315229 (1967-04-01), Smithline
patent: 3705383 (1972-12-01), Frayer
patent: 3932839 (1976-01-01), Stephens

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