Method and agent for the immunological determination of enzymes

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 21, G01N 3354, C12Q 142

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active

043662420

ABSTRACT:
A process for the quantitative immunological determination of an enzyme in a liquid, comprises incubating anti-enzyme antibodies which are adsorbed on a water-insoluble carrier, with the sample solution containing the enzyme, whereby the enzyme bonds to the antibodies but retains a reproducible proportion of its activity in its complex with the antibodies; removing the sample solution; washing the antibody carrier, which is charged with enzyme; and determining the activity of the enzyme bonded to the antibodies.

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