Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-02-07
2006-02-07
Coe, Susan D. (Department: 1655)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
Reexamination Certificate
active
06994973
ABSTRACT:
The invention relates to a dry chemical method and a test strip for determining calcium and magnesium in aqueous samples. Said test strip contains o-cresolphthalein complex (o-CPC; CAS Registry No. 2411-89-4) as a colour-causing reagent in a buffer system with a pH value of below 8.5. By using in particular an aqueous ethanolic imidazole buffer with a pH value of 6.75, conditions can be set up in such a way that the o-CPC has an identical molar sensitivity with respect to Ca and Mg. The total water hardness of aqueous samples can thus be easily determined.
REFERENCES:
patent: 3822116 (1974-07-01), Morin
patent: 3938954 (1976-02-01), Stavropoulos et al.
patent: 4871678 (1989-10-01), Wahl et al.
patent: 4904605 (1990-02-01), O'Brien et al.
patent: 5302346 (1994-04-01), Vogel et al.
patent: 62064951 (1987-03-01), None
Krenn Karl-Dieter
Tanzer Dieter
Coe Susan D.
McCormick-Ewoldt S. B.
Merck Patent GmbH
Millen White Zelano & Branigan P.C.
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