Method and additives for improving the quality and shelf life of

Drug – bio-affecting and body treating compositions – Fermentate of unknown chemical structure – Having a known elemental analysis

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435 2, A61K 3514, A61K 3518

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047740884

ABSTRACT:
A method for improving the quality and/or increasing the shelf-life of whole blood and red blood cell concentrates during storage thereof comprising manipulating the activities of the key red cell enzymes involved in the biosynthesis and degradation of 2,3-DPG (DPGM, DPGP, PGP) and in regulation of glycolysis (PK and PFK). The enzymes are manipulated, i.e. activated or inhibited, either singly or in combination. Such manipulations are achieved by adding to the whole blood or red blood cell concentrates an effective amount of one or more compounds which primarily inhibit the activity of PK and secondarily inhibit the activity of DPG phosphatase and maintain those of phosphofructokinase, phosphoglycolate phosphatase and DPG mutase. Among the compounds which can be used are L-amino acids, free fatty acids, glycolytic intermediates including analogs and derivatives of phosphoenolpyruvate, and free bases, inhibitors of DPGP as well as structural analogs and derivatives of all of the above compounds. Another compound which can be used to improve the quality of stored blood is a pyrophosphate compound.

REFERENCES:
patent: 3925153 (1975-12-01), Laborit
patent: 4112070 (1978-09-01), Harmening
patent: 4476221 (1984-10-01), Kane et al.
patent: 4572899 (1986-02-01), Walker et al.
patent: 4609372 (1986-09-01), Carmen et al.
Merck Index-ninth edition (1976), p. 1039.

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