Method and a kit for diagnosing plant diseases

Chemistry: analytical and immunological testing – Including sample preparation – Liberation or purification of sample or separation of...

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422 58, 422 61, 436174, 436178, 436503, 436518, G01N 102

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active

052197611

ABSTRACT:
A method and a kit for testing a plant for a suspected antigen. A sample of the plant is taken and rubbed between first and second pads to extract sap from the sample and to collect the sap on the first pad. The sap is transferred to a liquid solution, and that solution is tested for the suspected antigen. This may be done by contacting the solution with a tag antibody and with a carrier having a capture antibody so that a tag antibody-antigen-capture antibody complex forms on the carrier if the suspected antigen is in the liquid solution. The carrier is then tested for the tag antibody. The kit preferably includes the first and second pads, the carrier, containers to hold the liquid solution and the tag antibody, and a device or reagent to test the carrier for the tag antibody.

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