Method and a hybrid promoter for controlling exogenous gene tran

Details Method and a hybrid promoter for controlling exogenous gene tran Method and a hybrid promoter for controlling exogenous gene tran

1991-10-01

1994-05-10

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 536 241, 935 28, 935 37, C12N 1500, C12N 1563, C12N 1581, C12P 2100

Patent

active

053106601

ABSTRACT:
A hybrid promoter for controlling exogenous gene transcription is constructed by insertion of a UAS.sub.G into a GPD portable promoter. The resulting hybrid promoter is placed upstream of an exogenous gene in a hybrid yeast-bacterial plasmid, which is used to transform yeast cells. Due to the regulation imparted to the GPD promoter by the UAS.sub.G, transcription of the exogenous gene, and hence production of the exogenous gene product, may be regulated by controlling the composition of the carbon source in the yeast culture medium. Specifically, glucose is used to repress transcription and galactose is used to induce transcription.

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