Method and a device for the evaluation of biopolymer fitness

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C356S036000, C356S302000, C356S306000, C356S311000, C356S319000, C356S320000, C356S335000, C422S051000, C422S051000, C422S091000, C422S129000, C422S186000, C435S007100, C435S287100, C435S287200, C435S288700, C435S808000, C436S501000, C436S517000, C436S518000, C436S034000, C436S043000, C436S805000, C436S172000

Reexamination Certificate

active

10435674

ABSTRACT:
A Method for identifying one or a small number of molecules, especially in a dilution of ≦1 μM, using laser excited FCS with measuring times ≦500 ms and short diffusion paths of the molecules to be analyzed, wherein the measurement is performed in small volume units of preferably ≦10−14l, by determining material-specific parameters which are determined by luminescence measurements of molecules to be examined.The device which can be preferably used for performing the method according to the invention is a per se known system of microscope optics for laser focusing for fluorescence excitation in a small measuring compartment of a very diluted solution and for imaging the emitted light in the subsequent measurement through confocal imaging wherein at least one system of optics with high numerical aperture of preferably ≧1.2 N.A. is employed, the light quantity is limited by a confocally arranged pinhole aperture in the object plane behind the microscope objective, and the measuring compartment is positioned at a distance of between 0 and 1000 μm from the observation objective.

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