Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-07-10
2007-07-10
Chin, Christopher L. (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C356S036000, C356S302000, C356S306000, C356S311000, C356S319000, C356S320000, C356S335000, C422S051000, C422S051000, C422S091000, C422S129000, C422S186000, C435S007100, C435S287100, C435S287200, C435S288700, C435S808000, C436S501000, C436S517000, C436S518000, C436S034000, C436S043000, C436S805000, C436S172000
Reexamination Certificate
active
10435674
ABSTRACT:
A Method for identifying one or a small number of molecules, especially in a dilution of ≦1 μM, using laser excited FCS with measuring times ≦500 ms and short diffusion paths of the molecules to be analyzed, wherein the measurement is performed in small volume units of preferably ≦10−14l, by determining material-specific parameters which are determined by luminescence measurements of molecules to be examined.The device which can be preferably used for performing the method according to the invention is a per se known system of microscope optics for laser focusing for fluorescence excitation in a small measuring compartment of a very diluted solution and for imaging the emitted light in the subsequent measurement through confocal imaging wherein at least one system of optics with high numerical aperture of preferably ≧1.2 N.A. is employed, the light quantity is limited by a confocally arranged pinhole aperture in the object plane behind the microscope objective, and the measuring compartment is positioned at a distance of between 0 and 1000 μm from the observation objective.
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Brinkmeyer Michael
Eigen Manfred
Henco Karsten
Lehman Olaf
Mets Ulo
Chin Christopher L.
Jacobson & Holman PLLC
Olympus Corporation
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