Methanol inducible genes obtained from pichia and methods of use

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 68, 435 70, 4351723, 435320, 435255, 435 91, 935 28, 935 37, 935 6, 935 69, C12P 2100, C12N 1500, C12N 116, C12Q 168

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048085375

ABSTRACT:
A method for isolating and cloning methanol inducible genes from Pichia is taught. The regulatory regions are useful for the methanol regulated expression of heterologous genes.

REFERENCES:
Sibirny et al. Curr. Genet. vol. 8, pp. 107-114 Feb. 1984 "Identification of Regulating Genes of ribflum Permeaseant .alpha.-Glucosidase in the Least Pichia guillermondii".
Tuite et al. EMBO Vol. 1, pp. 603-608 1982 "Regulated High Efficiency Expression of Human Interference-Alpha in Succhammyceo cerevisiac".
Bennetzen et al. J. Biol. Chem vol. 257 (6), Mar. 25, 1982, pp. 3018-3025 "The Primary Structure of the Succanomyceo cerenisium Gene for Alcohol Dehydrobyenase I".
Kunze et al. Curr Aeret vol. 9 pp. 205-209, 1985 "Transformation of Cazdida Maltosa and Pichia guittiermardii by a Plasmid Containing Sacchanmyceo cerevisiuc Argy DNA".

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