Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Patent
1997-06-19
1999-10-12
Smith, Lynette F.
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
800278, 800295, 800288, 435 691, 435468, 435419, 4353201, 536 232, 536 241, 536 237, C12N 1529, C12N 1582, A01H 400, A01H 500
Patent
active
059657960
ABSTRACT:
The present invention provides nucleic acid sequences encoding a metal ion resistance protein, which are expressible in plant cells. The metal resistance protein provides for the enzymatic reduction of metal ions including but not limited to divalent Cu, divalent mercury, trivalent gold, divalent cadmium, lead ions and monovalent silver ions. Transgenic plants which express these coding sequences exhibit increased resistance to metal ions in the environment as compared with plants which have not been so genetically modified. Transgenic plants with improved resistance to organometals including alkylmercury compounds, among others, are provided by the further inclusion of plant-expressible organometal lyase coding sequences, as specifically exemplified by the plant-expressible merB coding sequence. Furthermore, these transgenic plants which have been genetically modified to express the metal resistance coding sequences of the present invention can participate in the bioremediation of metal contamination via the enzymatic reduction of metal ions. Transgenic plants resistant to organometals can further mediate remediation of organic metal compounds, for example, alkylmetal compounds including but not limited to methyl mercury, methyl lead compounds, methyl cadmium and methyl arsenic compounds, in the environment by causing the freeing of mercuric or other metal ions and the reduction of the ionic mercury or other metal ions to the less toxic elemental mercury or other metals.
REFERENCES:
patent: 4849355 (1989-07-01), Wong
patent: 5364451 (1994-11-01), Raskin et al.
patent: 5380381 (1995-01-01), Adang et al.
patent: 5668294 (1997-09-01), Meagher et al.
Thompson. PhD. Dissertation, University of Georgia, 1990.
Murray et al. Nucleic Acids Research vol. 17 No. 2, 1989.
Summers et al. Ann. Rev. Microbiol. 40: 607-634, 1986.
Stack, N.M. (1992) The Reconstruction of the Bacterial Gene merA,, BS Thesis, University of Georgia.
Meagher, R.B. (1994) "Phyto-remediadtion of heavy metal ion toxicity: A highly modified bacterial MerA gene confers mercuric ion resistance to transgenic Arabidopsis plants," Abstract of presentation to Dept. of Energy Phyto-remediation Research Workshop, Santa Rosa, California, Jul. 25-26, 1994.
Wilde et al. (1994) In vitro Cellular & Developmental Biology Animal 30A (3 part 2), p. 60.
Rugh et al. (1994) "Ionic Mercury Detoxification by Transgenic Plants," poster abstract presented at American Society of Plant Physiology Annual Meeting, Portland, Oregon, Jul. 30-Aug. 3, 1994.
Thompson, D.M. (1990) Transcriptional and Post-transcriptional Regulation of the Genes Encoding the Small Subunit of Rubulose-1, 5-Bisphosphate Carboxylase, Ph.D. Thesis, University of Georgia, Athens, Georgia.
Grill et al. (1987) Proc. Natl. Acad. Sci. USA 84:439-443.
Lefebvre et al. (1987) Biotechnology 5:1053-1056.
Gilbert and Summers (1988) Plasmid 20:127-136.
Begley et al. (1986) Biochemistry 25:7186-7192.
Begley et al. (1986) Biochemistry 25:7192-7200.
Summers, A.O. (1986) Ann. Rev. Microbiology 40:607-634.
Summers and Sugarman (1974) Journal of Bacteriology 119:242-249.
Rinderle et al. (1983) Biochemistry 22:869-876.
Barrineau et al. (1984) Journal of Molecular and Applied Genetics 2:601-619.
McClelland and Ivarie (1982) Nucleic Acids Research 10:7865-7877.
Murray et al. (1989) Nucleic Acids Research 17:477-494.
Brown et al. (1983) Biochemistry 22: 4089-4095.
Misra et al. (1985) Gene 34:253-262.
Stormo et al. (1982) Nucleic Acids Res. 10:2971-2996.
Heidecker and Messing (1986) Ann. Rev. Plant Physiol. 37:439-466.
Foster, T.J. (1983) Microbiol. Rev. 47:361-409.
Rensing et al. (1992) Journal of Bacteriology 174:1288-1292.
Scheller et al. (1987) Plant Physiology 85:1031-1035.
Barkay et al. (1992) Biodegradation 3:147-159.
Brunker et al. (1966) Mol. Gen. Genet. 251:307-315.
Clark et al. (1977) J. of Bacteriology 132(1):186-196.
Dacey, J.W. (1980) Science 210:1017-1019.
Dacey, J.W. (1981) Ecology 62:1137.
Gilbert, et al. (1988) Plasmid 20:127-136.
Ogawa et al. (1984) Gene 32:311-320.
Robinson & Tuovinen (1984) Microbiol Reviews 48:95-124.
Rugh et al. (1996) Proc. Natl. Acad. Sci. USA 93:3182-3187.
Meagher Richard Brian
Rugh Clayton L.
Summers Anne O.
Haas Thomas
Smith Lynette F.
University of Georgia Research Foundation Inc.
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