Chemistry: analytical and immunological testing – Metal or metal containing – Present in biological fluids
Patent
1996-08-28
1998-09-29
Ceperley, Mary E.
Chemistry: analytical and immunological testing
Metal or metal containing
Present in biological fluids
436 79, 436 80, 436 81, 436 83, 436 84, 436533, 436534, 436909, G01N 3320, G01N 35546
Patent
active
058145214
ABSTRACT:
The present invention relates to a method for the determination of polyvalent metal ions using a sandwich aggregation assay. One or more chelating agents capable of forming at least 2:1 or higher ratio stoichiometry complexes with polyvalent metal ions are attached to a suitable carrier such as latex particles through covalent bonds or by non-covalent interactions, such as hydophobic interactions. Upon complexation with the metal ions, the carriers of the complexing agent aggregate, causing an increase in light absorbance or light scattering which is proportional to the concentration of metal ions in the test sample. The measurement of the light absorbance or light scattering by a suitable instrument, such as a spectrophotometer or nephelometer provides a means for measuring the concentration of metal ions.
REFERENCES:
patent: 4080171 (1978-03-01), Sano et al.
patent: 5407831 (1995-04-01), Jing
patent: 5459040 (1995-10-01), Hammock et al.
R. Schall et al., Clin. Chem., vol. 27, No. 7, pp. 1157-1164 (1981).
L. Bangs, Pure & Applied Chem., vol. 68, No. 10 pp. 1873-1879 (1996).
Aggarwal, et al., Clin. Chem. vol. 40, No. 8, pp. 1494-1502 (1994).
Schneider, et al., Analyst, vol. 118 pp. 993-936, (Sep. 1993).
Lerchi, et al., Anal. Chem. vol. 64 pp. 1534-1540, (1992).
Chapoteau Eddy
Craine Jonathan
Czech Bronislaw P.
Kumar Anand
Leong Koon-Wah
Bayer Corporation
Ceperley Mary E.
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