Metal chelating compositions for protein purification,...

Details Metal chelating compositions for protein purification,... Metal chelating compositions for protein purification,...

2006-04-25

2006-04-25

Cole, Monique T. (Department: 1743)

Compositions

Miscellaneous

C252S390000, C436S166000, C436S086000, C562S512000, C562S553000, C562S571000

Reexamination Certificate

active

07033520

ABSTRACT:
A metal chelating composition having the formula:whereinQ is a carrier;S1is a spacer;L is -A-T-CH(X)— or —C(═O)—;A is an ether, thioether, selenoether, or amide linkage;T is a bond or substituted or unsubstituted alkyl or alkenyl;X is —(CH2)kCH3, —(CH2)kCOOH, —(CH2)kSO3H, —(CH2)kPO3H2, —(CH2)kN(J)2, or —(CH2)kP(J)2, preferably —(CH2)kCOOH or —(CH2)kSO3H;k is an integer from 0 to 2;J is hydrocarbyl or substituted hydrocarbyl;Y is —COOH, —H, —SO3H, —PO3H2, —N(J)2, or —P(J)2, preferably, —COOH;Z is —COOH, —H, —SO3H, —PO3H2, —N(J)2, or —P(J)2, preferably, —COOH; andi is an integer from 0 to 4, preferably 1 or 2.

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