Metal binding proteins, recombinant host cells and methods

Details Metal binding proteins, recombinant host cells and methods Metal binding proteins, recombinant host cells and methods

2001-10-12

2004-06-15

Nelson, Amy J. (Department: 1638)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S419000, C435S320100, C536S023100

Reexamination Certificate

active

06750042

ABSTRACT:

BACKGROUND OF THE INVENTION
The field of the present invention is the area of molecular biology, in particular as related to a metal-binding protein produced through recombinant DNA technology.
One of the best-characterized mercury resistance (mer) operons is located on transposon Tn21 from the
Shigella flexneri
IncFII plasmid R100. This operon consists of five structural genes—merT, merP, merC, merA, and merD—and a regulatory gene, merR (FIG.
1
). MerT, MerP, and MerC are involved in the transport of Hg(II) into the cell. MerA is a cytosolic, NADPH-dependent, flavin adenine dinucleotide-containing oxidoreductase which reduces Hg(II) to Hg(0). The merR gene is transcribed in the direction opposite from that of the structural genes. Its product, MerR, represses expression of the merTPCAD genes in the absence of Hg(II), activates their expression in the presence of Hg(II), and represses its own expression in the presence or absence of Hg(II). MerD plays a minor role in regulation, possibly as an antagonist of MerR, which reestablishes repression of merTPCAD once Hg(II) has teen reduced to Hg(0).
Extensive genetic and biochemical data indicate that the protein contains three domains [Ross; et al. (1989)
J. Bacteriol.
171:4009-4018; Shewchuk et al. (1989)
Biochemistry
28:2340-2344; Summers, A. O. (1992)
J. Bacteriol.
174:3097-3101]: a helix-turn-helix DNA-binding domain from L10 to R29 [Brennan et al. (1989)
J. Biol. Chem.
264:1903-1906; Livrelli et al. (1993)
J. Biol. Chem.
268:2623-2631]; a “coupling” domain from K30 to H81 which may convey the status of the Hg(II) binding site to the DNA binding site [Comess et al. (1994)
Biochemistry
33:4175-4186; Helmann et al. (1990)
Science
247:946-948]; and a long helical region from C82 to C117 which constitutes both the dimer interface and, with the loop containing C126, the Hg(II)-binding domain [Zeng et al. (1998)
Biochemistry
37:15885-15895].
The MerR homodimer binds Hg(II) by using the thiols of three conserved cysteines: cysteine 82 (C82) from one monomer and cysteines 117 and 126 (C117 and C126) from the other monomer. These ligands form a novel planar tricoordinate complex with Hg(II) [Helman et al. (1990) supra; O'Halloran, T. V. (1993)
Science
261:715-725; Utschig et al. (1995)
Science
26:380-385; Wright et al. (1990)
J. Am. Chem. Soc.
112:2434-2435]. Upon binding Hg(II), MerR undergoes a conformational change that leads to an underwinding of the P
r
region and thereby enables RNA polymerase to form an open complex. Curiously, although the MerR homodimer contains two potential Hg(II) binding sites, the purified protein binds only one Hg(II) per dimer. Moreover, although the two other group 12 metals, Zn(II) and Cd(II), also form stable complexes with protein thiols [Boulanger et al. (1983)
Proc. Natl. Acad. Sci. USA
80:1501-1505; Furey et al. (1986)
Science
231:704-708; Santos et al. (1991)
J. Am. Chem. Soc.
113:469-474; Utschig et al. (1993)
Methods Enzymol.
226:71-97], purified MerR binds Hg(II) preferentially even in the presence of a 1,000-fold excess of Cd(II) or Zn(II) [Shewchuk et al. (1989)
Biochemistry
28:2331-2339] and also requires 100- to 1,000-fold higher concentrations of these metals for transcriptional activation [Ralston et al. (1990)
Proc. Natl. Acad. Sci. USA
87:3846-3850.
Although several studies have described mutants altered in either repression or activation, no genetic study of merR has explicitly addressed the basis of its metal specificity prior to Caguiat et al. (1999)
J. Bacteriol.
181:3462-3471. Caguiat et al. report variants of MerR with an altered response to a metal; the properties and locations of these mutations shed light on the basis for metal-provoked activation by MerR. That also led those authors to examine the possible occurrence of similar secondary-structure elements in other members of the MerR family, and our findings in the latter regard indicate conservation of a structural domain in a subset of this family.
There is a long felt need in the art for compositions and methods useful in the remediation of materials contaminated with heavy metal ions, especially mercury and cadmium. The present invention provides artificial metal binding proteins, coding sequences, recombinant DNA molecules, recombinant host cells and methods for production of the artificial metal binding proteins.
SUMMARY OF THE INVENTION
The present invention provides DNA sequences encoding artificial metal binding proteins, which are termed chelons herein, and DNA sequences encoding the mercury binding protein MerR (derived from Tn21), vectors comprising those sequences operably linked to promoters functional in particular host cells of choice, recombinant host cells expressing the chelon proteins or MerR protein and methods of producing the chelon proteins and MerR protein recombinantly. Host cells include, but are not limited to, bacterial cells (e.g.,
Escherichia coli, Bacillus subtilis
, Pseudomonas species, etc.), yeast cells (e.g.,
Sacciaromyces cerevisiae
or
Pichia pastoris
), fungi (e.g., Aspergillus species,
Trichoderma reesei
), plant cells (e.g., Arabidopsis, tobacco, petunia, Populus species, Salix species, among others) and animal cells such as CHO cells or avian cells.
The present invention further provides chelon proteins which bind heavy metal ions, including but not limited to divalent cadmium and mercury ions, as well as cobalt, copper, lead, nickel and zinc cations, with relatively high affinity. The amino acid sequence of a mercury-specific chelon is disclosed in Table 1D, and the nucleotide sequence encoding it is present in Table 1C. Additional chelons which bind both mercury and cadmium ions with high affinity are disclosed in Table 2. The chelon proteins can be within cells or on the surfaces of the cells in which they were produced, for example, for use in methods of concentrating heavy metal ions from a contaminated aqueous environment, or waste stream, or the chelon proteins can be immobilized onto a solid support for use in removal of heavy metal ions from a contaminated aqueous medium. The MerR protein can also be produced for in situ metal ion binding, or it can be purified and immobilized to a support material.
Also within the scope of the present invention are methods of removing heavy metal ions from contaminated aqueous solutions, waste streams or contaminated environments, for example, using immobilized chelon or MerR proteins, immobilized cells containing the MerR or chelon proteins or using whole plants to take up, sequester and concentrate the heavy metal ions from contaminated soil, ground water, hydroponic solutions, irrigation water or waste streams. Selective expression of the MerR or chelon proteins in above-ground plant parts (such as stems and leaves) allows the harvesting of the plant material in which the heavy metal ions have been sequestered and concentrated. Those plant parts can then be disposed of or processed in an environmentally appropriate manner.


REFERENCES:
patent: 5506121 (1996-04-01), Skerra et al.
patent: 5972656 (1999-10-01), Lopez et al.
patent: 0 585 652 (1994-09-01), None
Boulanger, Y. et al., “Model for mammalian metallothionein structure”(Mar. 1983) Proc. Natl. Acad. Sci. USA 80:1501-1505.
Brennan, R.G. and Matthews, B.W., “The Helix-Turn-Helix DNA Binding Motif” (Feb. 5, 1989) J. Biol. Chem. 264(4):1903-1906.
Caguiat, J.J. et al., “Cd(II)-Responsive and Constitutive Mutants Implicate A Novel Domain in MerR”(Jun. 1999)J. Bacteriol. 181(11):3462-3471.
Caguiat, J.J. and Summers, A.O., “Single Residue Changes Confer an Enhanced Response by MerR to Cd(II)” Abstracts of the General Meeting of the American Society for Microbiology (1998) 98p278.
Comess, K.L. et al., “Construction of a Synthetic Gene for the Metalloregulatory Protein MerR and Analysis of Regionally Mutated Proteins for Transcriptional Regulation” (1994) Biochemistry 33(14):4175-4786.
Engst, S. and Miller S. M., “Alternative Routes for Entry of HgX2into the Active Site

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