Metal-binding motif compositions and methods

Details Metal-binding motif compositions and methods Metal-binding motif compositions and methods

2006-05-30

2010-02-09

Kam, Chih-Min (Department: 1656)

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

C514S006900, C514S400000, C435S069100, C435S320100, C435S325000

Reexamination Certificate

active

07659362

ABSTRACT:
Disclosed herein are recombinant copper or zinc binding polypeptides containing at least one copper or zinc binding motif and a sequence heterologous to it. Also disclosed is a method to reduce the concentration of a free copper or zinc ion in a substrate by contacting it with a recombinant polypeptide containing a copper or zinc binding motif. A further method relates to reducing the concentration of a free copper or zinc ion in a substrate by contacting it with a host cell that expresses a recombinant polypeptide containing a copper or zinc binding motif.

REFERENCES:
patent: 2006/0029942 (2006-02-01), Yang
patent: WO 01/72771 (2001-10-01), None
Benson et al. “Rational design of nascent metalloenzymes”. PNAS 97(12):6292-6297, Jun. 2000.
She et al. “Identification of metal-binding proteins in human hepatoma lines by immobilized metal affinity chromatography and mass spectrometry”. Molecular & Cellular Proteomics2:1306-1318, 2003.
Baltzer et al. “Emerging principles of de novo catalyst design” Current Opinion in Biotechnology 12:355-360, 2001.
Ghosh et al. “Probing metal—protein interactions using a de novo design approach”. Current Opinion in Chemical Biology 9:97-103, 2005.
Lu. “Design and engineering of metalloproteins containing unnatural amino acids or non-native metal-containing cofactors”. Current Opinion in Chemical Biology 9:118-126, 2005.
Arnesano et al. “The unusually stable quaternary structure of human Cu,Zn-superoxide dismutase 1 is controlled by both metal occupancy and disulfide status”, Journal of Biological Chemistry 279(46):47998-48003, 2004.
Rojas et al. “De novo heme proteins from designed combinatorial libraries”. Protein Science 6:2512-2524, 1997.
Moffet et al. “Peroxidase activity in heme proteins derived from a designed combinatorial library”. J. Am. Chem. Soc. 122;7612-7613, 2000.
Wei et al. “Enzyme-like proteins from an unselected library of designed amino acid sequences”. Protein Engineering, Design & Selection 17(1):67-75, 2004.
Pinto et al. “Construction of a catalytically active iron superoxide dismutase by rational protein design”. Proc. Natl. Acad. Sci. USA 94:5562-5567, May 1997.
Coldren et al. “The rational design and construction of a cuboidal iron-sulfur protein”. Proc. Natl. Acad. Sci. USA 94:6635-6640, Jun. 1997.
Benson et al. “The development of new biotechnologies using metalloprotein design”. Curr. Opin Biotechnol. 9(4):370-376, Aug. 1998. Abstract only.
Ueno et al. “Coordinated design of cofactor and active site structures in development of new protein catalysts”. J. Am. Chem. Soc. 127(18):6556-6562, May 2005. Abstract only.
Thomas et al. “Artificial metalloenzymes: proteins as hosts for enantioselective catalysis”. Chem. Soc. Rev. 34(4):337-346, Apr. 2005. Abstract only.

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