Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi
Reexamination Certificate
2006-03-28
2006-03-28
Priebe, Scott D. (Department: 1633)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Fungi
C435S254210, C435S254230, C435S161000, C435S471000, C435S483000
Reexamination Certificate
active
07018829
ABSTRACT:
A recombinant microbial cell comprises at least one increased expressible enzyme activity controlling anabolic metabolism of ammonia as a nutrient source, the increased enzyme activity being an NADH-dependent activity catalysing the reaction(a) 2-oxoglutarate+NH3+NADH→glutamate+NAD or being an activity catalysing the reaction(b) 2-oxoglutarate+glutamine+NADH→2 glutamate+NAD or being an activity catalysing the reaction(c) glutamate+NH3+ATP→glutamine+ADP+Pi.The increased enzyme activity is encoded by a nucleic acid coding sequence linked to an expression signal not natively associated with the nucleic acid coding sequence and is increased as compared to the expression of the enzyme activity when the nucleic acid coding sequence is associated with its native expression signal.
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Kielland-Brandt Morten C.
Nielsen Jens
Nissen Torben Lauesgaard
Burkhart Mike
Cooper Iver P.
Fluxome Sciences A/S
Priebe Scott D.
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