Metabolically engineered microbial cell with an altered...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S254210, C435S254230, C435S161000, C435S471000, C435S483000

Reexamination Certificate

active

07018829

ABSTRACT:
A recombinant microbial cell comprises at least one increased expressible enzyme activity controlling anabolic metabolism of ammonia as a nutrient source, the increased enzyme activity being an NADH-dependent activity catalysing the reaction(a) 2-oxoglutarate+NH3+NADH→glutamate+NAD or being an activity catalysing the reaction(b) 2-oxoglutarate+glutamine+NADH→2 glutamate+NAD or being an activity catalysing the reaction(c) glutamate+NH3+ATP→glutamine+ADP+Pi.The increased enzyme activity is encoded by a nucleic acid coding sequence linked to an expression signal not natively associated with the nucleic acid coding sequence and is increased as compared to the expression of the enzyme activity when the nucleic acid coding sequence is associated with its native expression signal.

REFERENCES:
patent: 5830716 (1998-11-01), Kojima et al.
patent: 0733712 (1996-09-01), None
patent: WO 8703006 (1987-05-01), None
patent: WO 9810089 (1998-03-01), None
patent: WO 9818909 (1998-05-01), None
Avenda{overscore (n)}o, et al., “GDH3 Encodes a Glutamate Dehydrogenase Isozyme, a Previously Unrecognized Route for Glutamate Bioxynthesis inSaccharomyces cerevisiae,” Journal of Bacteriology, 1997, 5594-5597, 179(17).
Voordouw, et al., “Structure of Pyridine Nucleotide Transhydrogenase fromAzotobacter vinelandii,” Eur. J. Biochem., 1979, 447-454, 98.
Flores-Samaniego, et al., “Glutamine Synthesis is a Regulatory Signal Controlling Glucose Catabolism inSaccharomyces cerevisiae,” Journal of Bacteriology, 1993, 7705-7706, 175(23).
Wakisaka, et al., “Characteristics and efficiency of Glutamine Production by Coupling of a Bacterial Glutamine Synthetase Reaction with the Alcoholic Fermentation System of Baker's Yeast,” Applied Environmental Microbiology, 1998, 2952-2957, 64(8).
Boonstra, et al., “Sequence of the Soluble Pyridine Nucleotide Transhydrogenase ofAzotobacter vinelandii,” 1999.
Anderlund, et al., “Expression of theEscherichia colipntA and pntB Genes, Encoding Nicotinamide Nucleotide Transhydrogenase, inSaccharomyces cerevisiaeand Its Effect on Product Formation during Anaerobic Glucose Fermentation,” Applied and Environmental Microbiology, 1999, 2333-2340, 65(6).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Metabolically engineered microbial cell with an altered... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Metabolically engineered microbial cell with an altered..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Metabolically engineered microbial cell with an altered... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3591609

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.