Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Reexamination Certificate
2008-07-01
2008-07-01
Vogel, Nancy (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
C435S144000, C435S161000, C435S254210
Reexamination Certificate
active
07393669
ABSTRACT:
A recombinant prototrophic micro-organism such as a yeast is provided which over-expresses the activity of an enzyme catalysing the conversion of glucose-1 phosphate to glucose-6 phosphate in the galactose uptake and metabolism pathway. Suitably the increased enzyme activity is due to over-expression of the gene PGM2. This produces increased uptake of galactose and increased ethanol production when the organism is grown on a galactose containing medium.
REFERENCES:
patent: 6747137 (2004-06-01), Weinstock et al.
Ostergaard, et al, Increasing galactose consumption bySaccharomyces cerevisiaethrough metabolic engineering of the GAL gene regulatory network, Nature Biotechnology 18:1283-1286, 2000.
Oh D and Hopper JE, Transcription of a Yeast Phosphoglucomutase Isozyme Gene is Galactose Inducible and Glucose Repressible, Molecular and Cellular Biology 10(4):1415-1422, 1990.
Hofmann, M., et al. Characterization of the essential yeast gene encoding N-acetylglucosamine-phosphate mutase. Eur. J. Biochem. 221:741-747, 1994.
Hoffmann, B., et al. Development and metabolic regulation of the phosphoglucomutase-encoding gene, pgmB, ofAspergillus nidulans. Mol. Gen. Genet. 262:1001-1011, 2000.
Bergmeyer et al “Samples, reagents, assessment of results,”Methods of enzymatic analysis2 (2): 278-9, 326-7 (1983).
Bro et al “Metabolic engineering of the galactose utilization pathway inSaccharomyces cerevisiaeusing DNA arrays,”3rdInternational Conference on Systems BiologyKurolinska Institutet, Sockholm Sweden (Dec. 13-15, 2002).
Bro et al “The Impact of DNA arrays on Metabolic Engineering,”Esbes 4 SymposiumDelft University of Technology, The Netherlands (Aug. 28-31, 2002).
Daugherty et al “Purification and Properties of Phosphoglucomutase from Fleischmann's Yeast”,Eur. J. Biochem. 57: 115-128 (1975).
Dijken et al “An interlaboratory comparison of physiological and genetic properties of fourSaccharomyces cerevisiaestrains” ,Enzyme and Microbial Technology26: 706-714 (2000).
Elsevier et al “Heterodimer formation and activity in the human enzyme glactose-1-phosphate uridylytransferase”,Proc. Natl. Acad. Sci. USAGenetics 93: 7166-7171 (Jul. 1996).
Frolova et al “Binding of the glucose-dependent Mig 1p repressor to theGAL1andGAL4promoters in vivo: regulation by glucose and chromatin structure”,Nucleic Acids Research27 (5): 1350-1358 (1999).
Ideker et al “Integrated Genomic and Proteomic Analyses of a Systematically Perturbed Metabolic Network”,Science292: 929-934 (May 4, 2001).
Johnston “A Model Fungal Gene Regulatory Mechanism: theGALGenes ofSaccharomyces cerevisiae”, S. cerevisiae Gal Genes51 (4): 458-476 (1987).
Johnston et al “Regulation of Carbon and Phosphate Utilization”,The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression2, Chapter 5: 193-281 (1992).
Keleher et al “Ssn6-Tup1 Is a General Repressor of Transciption in Yeast”,Cell68: 709-719 (Feb. 21, 1992).
Klein et al “Glucose control inSaccharomyces cerevisiae: the role ofMIG1in metabolic functions”,Microbiology144: 13-24 (1998).
Lowry et al “Protein Measurement with the Folin Phenol Reagent”,J. Biol. Chem. 193: 265-275 (1951).
Masuda et al “Phosphoglucomutase Is an in Vivo Lithium Target in Yeast”,The Journal of Biological Chemistry276 (41): 37794-37801 (Oct. 12, 2001).
Melcher “Galactose Metabolism inSaccharomyces cerevisiae: A Paradigm for Eukaryotic Gene Regulation”,In Yeast Sugar Metabolism: Biochemistry, genetics, biotechnology, and applicationsChapter 14: 235-269.
Mizoguchi et al “Erythrocyte galactokinase assay with high performance liquid chromatography”,Clinica Chimica Acta216: 145-151 (1993).
Møller et al “Steady-state and transient-state analyses of aerobic fermentation inSaccharomyces kluyven”, FEMS Yeast Research2: 233-244 (2002).
Nielsen “Metabolic engineering”,Appl. Microbiol. Biotechnol. 55: 263-283 (2001).
Oh “Transcription of a Yeast Phosphoglucomutase Isozyme Gene Is Galactose Inducible and Glucose Repressible”,Molecular and Cellular Biology10 (4): 1415-1422 (1990).
Ostergaard et al “Increasing glactose consumption bySaccharomyces cerevisiaethrough metabolic engineering of theGALgene regulatory network”,Nature Biotechnology18: 1283-1286 (Dec. 2000).
Platt et al “The yeast galactose genetic switch is mediated by the formation of a Gal4p-Gal3p complex”,The EMBO Journal17 (14): 4086-4091 (1998).
Purnelle et al “Analysis of an 11•7 kb DNA Fragment of Chromosome XI Reveals a New tRNA Gene and Four New Open Reading Frames Including a Leucine Zipper Protein and a Homologue to the Yeast Mitochondrial Regulator ABF2”,Yeast10: 125-130 (1994).
Treitel et al “Repression by SSN6-TUP1 is directed by MIG1, a repressor/activator protein”,Proc. Natl. Acad. Sci. USA92: 3132-3136 (Apr. 1995).
Verduyn et al “Effect of Benzoic Acid on Metabolic Fluxes in Yeasts: A Continuous-Culture Study on the Regulation of Respiration and Alcoholic Fermentation”,Yeast8: 501-517 (1992).
Wieczorke et al “Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses inSaccharomyces cerevisiae”, FEBS Letters464: 123-128 (1999).
Zheng et al “The Cysteine-Peptidase Bleomycin Hydrolase Is a Member of the Galactose Regulon in Yeast”,The Journal of Biological Chemistry272 (48): 30350-30355 (Nov. 28, 1997).
Zhu et al “Global Analysis of Protein Activities Using Proteome Chips”,Science293: 2101-2104.
Bro Christoffer
Nielsen Jens
Regenberg Birgitte
Cooper Iver P.
Fluxome Sciences A/S
Vogel Nancy
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