Mesoscale polynucleotide amplification devices

Details Mesoscale polynucleotide amplification devices Mesoscale polynucleotide amplification devices

2011-02-22

2011-02-22

Bowers, Nathan A (Department: 1775)

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

C435S287200, C435S288300, C435S288400, C435S288600, C435S288700, C435S305100, C435S305200, C435S305300, C422S091000, C422S105000, C422S105000

Reexamination Certificate

active

07892819

ABSTRACT:
Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide amplification reaction. The devices are provided with a substrate microfabricated to include a polynucleotide amplification reaction, chamber, having at least one cross-sectional dimension of about 0.1 to 1000 μm. The device also includes at least one port in fluid communication with the reaction chamber, for introducing a sample to the chamber, for venting the chamber when necessary, and, optionally, for removing products or waste material from the device. The reaction chamber may be provided with reagents required for amplification of a preselected polynucleotide. The device also may include means for thermally regulating the contents of the reaction chamber, to amplify a preselected polynucleotide. Preferably, the reaction chamber is fabricated with a high surface to volume ratio, to facilitate thermal regulation.

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